chicken anti-mcherry antibody  (Novus Biologicals)

Journal: Nature Communications

Article Title: Sex-specific hypothalamic neural projection activity drives caregiving in mice

doi: 10.1038/s41467-025-59352-7

Figure Lengend Snippet:

Article Snippet: chicken anti-mCherry , Millipore, AB356481 , 1:1500.

Techniques:

Journal: Current Research in Neurobiology

Article Title: Regulation of perisomatic synapses from cholecystokinin basket interneurons through NrCAM and Ankyrin B

doi: 10.1016/j.crneur.2025.100150

Figure Lengend Snippet: Expression of tdTomato, NECAB1, and Parvalbumin in mPFC of Sncg-IRES-FlpO:tdT mice (P60) A. Immunofluorescent staining of tdT with dsRed antibodies in a representative confocal image (single optical section) captured at 20x. tdT labeled cells were present throughout the mPFC with greater abundance in layer II/III. Cortical layers I-VI are indicated. Image field of view was 1408 x 1408 pixels, or 3904 μm × 3904 μm. B. Control staining with secondary antibodies alone was low. C. Higher magnification of tdT + cells show neuronal morphology with labeled soma and processes (arrowheads). Bar = 10 μm. D. Intrinsic tdT fluorescence in mPFC layer 2/3 neurons without dsRed antibody staining is lower but visible in soma and processes (arrowheads). E. Time course of tdT expression at postnatal ages P34 (a), P42 (b), and P60 (c, d). Representative nonconfocal images of mPFC layer II/III stained for tdTomato using dsRed antibodies, or secondary antibodies alone, were obtained by EVOS microscopy. Images were captured with a 20X objective in a field of view of 2048× 1536 pixels, or 633 μm × 475 μm. F. Graph of cell density per mm2 by postnatal age. Significant differences in mean cell density∗ were determined by Anova and Tukey's post hoc analysis: P34 vs. P42: p = 0.005∗; P34 vs P60: p = 0.0002∗; P34-P60: p = 0.072, not significant. G. Double immunofluorescence staining for NECAB1 (blue) and tdT using mCherry antibodies (red) in confocal images (single optical sections) showed that a subset of mCherry + neurons co-expressed NECAB1 (∼25 %). Images were captured with a 20X objective in a field of view of 1024× 1024 pixels, or 319 μm × 319 μm. Representative regions of the confocal images are shown. Bars = 10 μm. Control staining with secondary antibodies alone was negative. H. Double immunofluorescence staining for parvalbumin (PV; blue) and tdTomato (anti-dsRed, red) indicated no detectable co-localization in representative confocal images. Images were captured with a 10X objective at 1024× 1024 pixels, or 639 μm × 639 μm. Representative regions of confocal images are shown. Bars = 50 μm.

Article Snippet: Rabbit anti-RFP antibody (Rockland 600-301-379S; 1:1000) and chicken anti-mCherry antibody (Aves Labs MC88697977; 1:250) were used to detect tdTomato.

Techniques: Expressing, Staining, Labeling, Control, Fluorescence, Microscopy, Double Immunofluorescence Staining